Polymorphism of the gene SHBG rs727428 associated with male infertility in a Vietnamese population
Author affiliations
DOI:
https://doi.org/10.15625/vjbt-21060Keywords:
Male infertility, PCR-RFLP, rs727428, SHBG, Vietnam.Abstract
Infertility is a complex disease, characterized by the incapability to achieve pregnancy after 24 months of regular unprotected sexual intercourse. Various studies have highlighted the role of sex hormone-related genes in contributing to the risk of male infertility. However, research on sex hormone-binding globulin (SHBG) – a glycoprotein involved in transporting sex hormones to target tissues, remains limited. Therefore, this study aimed to investigate whether SHBGrs727428 variant is associated with idiopathic male infertility in Vietnam. A total of 287 DNA samples (123 cases and 164 controls) were genotyped using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) method. The results showed that genotype frequencies conformed to the Hardy-Weinberg equilibrium. Importantly, an association between SHBG rs727428 and male infertility was identified in three models: additive model (TT genotype, OR = 0.428, 95%CI 0.197 – 0.885, p-value = 0.021), dominant model (TT genotypes, OR = 0.473, 95%CI 0.229 – 0.928, p-value= 0.029) and allele model (T allele, OR = 0.680, 95% CI: 0.481-0.95, p-value = 0.028). This study enhances our understanding of the role of genetic factors in male infertility among the Vietnamese population.
Downloads
References
An P., Rice T., Gagnon J., Hong Y., Leon A. S., Skinner J. S., et al. (2000). A genetic study of sex hormone—Binding globulin measured before and after a 20-week endurance exercise training program: The HERITAGE Family Study. Metabolism, 49(8), 1014-1020. https://doi.org/10.1053/meta.2000.7737
Aragon T. J. (2004). epitools: Epidemiology Tools (p. 0.5-10.1) [Dataset]. https://doi.org/10.32614/CRAN.package.epitools
Cui Y., Guo Y., Qiao S., Len L., Xie, Z., Chen H., et al. (2017). Correlation between SHBG gene polymorphism and male infertility in Han population of Henan province of China: A STROBE-compliant article. Medicine, 96(32), e7753. https://doi.org/10.1097/MD.0000000000007753
Eriksson A. L., Lorentzon M., Mellström D., Vandenput L., Swanson C., Andersson N., et al. (2006). SHBG gene promoter polymorphisms in men are associated with serum sex hormone-binding globulin, androgen and androgen metabolite levels, and hip bone mineral density. The Journal of Clinical Endocrinology & Metabolism, 91(12), 5029-5037. https://doi.org/10.1210/jc.2006-0679
Ge Y.-Z., Xu L.-W., Jia R.-P., Xu Z., Li W.-C., Wu R., et al. (2014). Association of polymorphisms in estrogen receptors (ESR1 and ESR2) with male infertility: A meta-analysis and systematic review. Journal of Assisted Reproduction and Genetics, 31(5), 601-611. https://doi.org/10.1007/s10815-014-0212-5
Graffelman J. (2015). Exploring diallelic genetic markers: The HardyWeinberg package. Journal of Statistical Software, 64(3), 1-23. https://doi.org/10.18637/jss.v064.i03
Hammond G. L. (1990). Molecular properties of corticosteroid binding globulin and the sex-steroid binding proteins. Endocrine Reviews, 11(1), 65-79. https://doi.org/10.1210/edrv-11-1-65
Hammond G. L. (2011). Diverse roles for sex hormone-binding globulin in reproduction. Biology of Reproduction, 85(3), 431-441. https://doi.org/10.1095/biolreprod.111.092593
Hekim N., Aydin M., Gunes S., & Asci R. (2022). Follicle‐stimulating hormone beta subunit and receptor variations in infertile men in Central Black Sea Region of Turkey. Andrologia, 54(5), e14383. https://doi.org/10.1111/and.14383
Krausz C., Escamilla A. R., & Chianese C. (2015). Genetics of male infertility: From research to clinic. Reproduction, 150(5), R159-R174. https://doi.org/10.1530/REP-15-0261
Krausz C., & Riera-Escamilla A. (2018). Genetics of male infertility. Nature Reviews Urology, 15(6), 369-384. https://doi.org/10.1038/s41585-018-0003-3
Kukuvitis A., Georgiou I., Bouba I., Tsirka A., Giannouli Ch., Yapijakis C., et al. (2002). Association of oestrogen receptor α polymorphisms and androgen receptor CAG trinucleotide repeats with male infertility: A study in 109 Greek infertile men. International Journal of Andrology, 25(3), 149-152. https://doi.org/10.1046/j.1365-2605.2002.00339.x
Laurent M. R., Hammond G. L., Blokland M., Jardí F., Antonio L., Dubois V., et al. (2016). Sex hormone-binding globulin regulation of androgen bioactivity in vivo: Validation of the free hormone hypothesis. Scientific Reports, 6(1), 35539. https://doi.org/10.1038/srep35539
Quy Vu P., Huu Dinh T., & Thuy Duong N. (2023). Assessing the association of ESR1 polymorphisms with male infertility in Vietnamese population. Vietnam Journal of Biotechnology, 21(4), 589-598. https://doi.org/10.15625/1811-4989/19500
Safarinejad M. R., Shafiei N., & Safarinejad S. (2010). Association of polymorphisms in the estrogen receptors alpha, and beta (ESR1, ESR2) with the occurrence of male infertility and semen parameters. The Journal of Steroid Biochemistry and Molecular Biology, 122(4), 193-203. https://doi.org/10.1016/j.jsbmb.2010.06.011
Safarinejad M. R., Shafiei N., & Safarinejad S. (2011). Association of the (TAAAA)n repeat and Asp327Asn polymorphisms in the sex hormone-binding globulin (SHBG) gene with idiopathic male infertility and relation to serum SHBG concentrations. The Journal of Steroid Biochemistry and Molecular Biology, 123(1–2), 37-45. https://doi.org/10.1016/j.jsbmb.2010.10.005
Selva D. M., Bassas L., Munell F., Mata A., Tekpetey F., Lewis J. G., et al. (2005). Human sperm sex hormone-binding globulin isoform: Characterization and measurement by time-resolved fluorescence immunoassay. The Journal of Clinical Endocrinology & Metabolism, 90(11), 6275-6282. https://doi.org/10.1210/jc.2005-1192
Turk A., Kopp P., Colangelo L. A., Urbanek M., Wood K., Liu K., et al. (2008). Associations of serum sex hormone binding globulin (SHBG) levels with SHBG gene polymorphisms in the CARDIA Male Hormone Study. American Journal of Epidemiology, 167(4), 412-418. https://doi.org/10.1093/aje/kwm332
Vanbillemont G., Bogaert V., De Bacquer D., Lapauw B., Goemaere S., Toye K., et al. (2009). Polymorphisms of the SHBG gene contribute to the interindividual variation of sex steroid hormone blood levels in young, middle‐aged and elderly men. Clinical Endocrinology, 70(2), 303-310. https://doi.org/10.1111/j.1365-2265.2008.03365.x
Wan Q., Xie Y., Zhou Y., & Shen X. (2021). Research progress on the relationship between sex hormone‐binding globulin and male reproductive system diseases. Andrologia, 53(1), e13893. https://doi.org/10.1111/and.13893
Wickham E. P., Ewens K. G., Legro R. S., Dunaif A., Nestler J. E., & Strauss J. F. (2011). Polymorphisms in the SHBG gene influence serum SHBG levels in women with polycystic ovary syndrome. The Journal of Clinical Endocrinology & Metabolism, 96(4), E719-E727. https://doi.org/10.1210/jc.2010-1842
Wu Q., Zhang J., Zhu P., Jiang W., Liu S., Ni M., et al. (2017). The susceptibility of FSHB -211G > T and FSHR G-29A, 919A > G, 2039A > G polymorphisms to men infertility: An association study and meta-analysis. BMC Medical Genetics, 18(1), 81. https://doi.org/10.1186/s12881-017-0441-4
Wu T.-S., & Hammond G. L. (2014). Naturally occurring mutants inform SHBG structure and function. Molecular Endocrinology, 28(7), 1026-1038. https://doi.org/10.1210/me.2014-1058
Xita N., & Tsatsoulis A. (2010). Genetic variants of sex hormone-binding globulin and their biological consequences. Molecular and Cellular Endocrinology, 316(1), 60-65. https://doi.org/10.1016/j.mce.2009.08.025
Zegers-Hochschild F., Adamson G. D., Dyer S., Racowsky C., De Mouzon J., Sokol R., et al. (2017). The international glossary on infertility and fertility care, 2017. Fertility and Sterility, 108(3), 393-406. https://doi.org/10.1016/j.fertnstert.2017.06.005
Downloads
Published
How to Cite
Issue
Section
Funding data
-
Ministry of Science and Technology
Grant numbers Grant No. DTDL.CN.60/19
